This hair mask moisture treatment should be used once or twice weekly but can be used up to five times. This is the final step of the 4-step system to increase hair growth. AnaGain stimulates specific signal molecules in the hair to reactivate and promote hair growth. AnaGain combined with 100mg of CBD per container helps to give your hair the nourishment and stimulation needed to grow fuller and thicker! After using the moisture treatment, follow with shampoo and conditioner. This product is intended to be used in a 4-step system but can be used separately.